Capto mmc impres binding capacity how to#

In addition, recommended screening strategies are presented where a sequential approach and Design of Experiments are used and discussed. However, the dynamic binding capacity (DBC) of Capto MMC ImpRes is relatively low under the selected condition, making the process less desirable for large-scale manufacturing.

Capto mmc impres binding capacity how to#

To use the full potential of Capto S, we show how to thoroughly screen the loading parameters, conductivity and pH. Capto S is a strong cation exchanger designed for capture and intermediate purification of recombinant proteins and as a second step in MAb purification. Previously launched products on this platform include Capto Q, Capto MMC and the MabSelect™ family. The medium combines high rigidity with high dynamic binding capacity (DBC) and fast mass transfer to allow faster purification, more flexible process design and maximum cost efficiency. High-resolution intermediate purification and polishing resin based on the well-established Capto platform with traditional ligands. For the polishing purification steps, ion exchange or multimodal resins are most commonly used. MabSelect PrismA for capture of the target. Thus, this new mixed-mode resin can be a potential substitute of Capto MMC ImpRes for post-Protein A purification. In addition, MMC Mustang shows good lot-to-lot consistency. This has recently been used in the development of a new cation exchange medium, Capto™ S, and gives a substantial increase in binding capacity compared to the corresponding media without dextran.Ĭapto S is a chromatography medium based on the novel high flow agarose platform. With a high binding capacity and small bead size, Capto SP ImpRes strong cation exchanger is a good choice for reliable and robust polishing steps in industrial purification. Capto MMC ImpRes resin is a multimodal cation exchange polishing resin designed for initial polishing after the protein A capture step in mAb workflows. The data suggest that in comparison with Capto MMC ImpRes, Diamond MMC Mustang exhibits comparable dynamic binding capacity (DBC) and resolution. flow uniformity required to obtain meaningful dynamic binding capacity measurements using. A technique for significantly improving the capacity and mass transfer properties of ion exchange media is modification of the porous base matrix with a surface extender such as dextran. anion exchange resins, Nuvia aPrime and Capto Adhere ImpRes. Increasing expression levels of recombinant proteins and the need for improved productivity and overall process economy puts extra demand on the next generation of chromatography media for the bio-pharmaceutical industry. Capto adhere/Capto adhere ImpRes: low pH (e.g., 0.1 to 0.5 M acetic acid) Capto MMC/Capto MMC ImpRes: high ionic strength solution (e.g.